FIREScript® RT cDNA synthesis MIX with Random primers

Properties

Sample type: RNA

Final product: cDNA (first strand)

Product format: Enzyme mix with all reagents included

Enzyme: FIRESript^® Reverse Transcriptase

Source: Purified from an E.coli strain that carries an overproducing plasmid containing a FIREScript^® Reverse Transcriptase gene.

Unit description: One unit is defined as the amount of enzyme that will incorporate 1 nmol of dTTP into acid-precipitable material in 10 minutes at 37°C using poly(rA)•oligo(dT) as a template in a total reaction volume of 50 μl.

Storage and dilution buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 7.5 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers.

Reagents

FIREScript^® Enzyme Mix (FIREScript^® RT and RiboGrip^® RNase inhibitor)

10x RT Reaction Premix without primers
RT reaction buffer with DTT and dNTPs

10x RT Reaction Premix with Random primers
RT reaction buffer with DTT, dNTPs and Random primers

10x RT Reaction Premix with Oligo (dT) primer
RT reaction buffer with DTT, dNTPs and Oligo (dT)

10x RT Reaction Premix with Oligo (dT) and Random primers RT reaction buffer with DTT, dNTPs, Oligo (dT) primer and Random primers

Water, nuclease free